Abstract: As an important component of city evolution, urban land redevelopment has an impact on transportation system. The current traffic impact analysis (TIA) is lack of a comprehensive component for non-motorized transportation under redevelopment. For a better guidance of land redevelopment and non-motorized transportation planning, it is necessary to evaluate the negative impact of redevelopment on non-motorized traffic in the TIA. In this paper, an evaluation framework for the impact analysis is built up. We organized the procedures and components of impact evaluation, and proposed the corresponding qualitative and quantitative evaluation indicators for non-motorized traffic under redevelopment. Level of service (LOS) and its criterion are employed for external impact evaluation, and level of safety, convenience, independence, and comfort which are four aspects of quality of service (QOS) are proposed to analyze the internal impact. The framework is applied to a redevelopment study in Shanghai, China. The case study results indicate that the redevelopment from a residential area to a mixed commercial area has a significant impact on non-motorized traffic. The potential negative impact from both external and internal traffic can be minimized by reasonable improvements in the internal land use design.